MiRNA Antagomir Negative Control

Components: This synthetic miRNA is based on the mature miRNA sequence. It does not contain the full precursor miRNA stem-loop. Directions for Use: Briefly centrifuge tubes containing miRNA antagomir to ensure that the miRNA pellet is located at the bottom of the tube. Dissolve miRNA antagomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water) . For example: dissolve 10 nmol miRNA antagomir to 20 μM using 500 μl DEPC H2O (or RNase-free water) . Pipette the solution up and down 3-5 times (or vortex briefly) . Briefly centrifuge tubes containing miRNA antagomir to ensure that the solution is collected at the bottom of the tube. Aliquot the miRNA antagomir into small volumes and store at ≤ -20°C. miRNA antagomir is stable (for 6 months under the specified storage condition) . For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times