Fibronectin, bovine Antibody
Rabbit polyclonal antibody to Fibronectin
Product Specifications
UniProt
P07589
Immunogen
Purified fibronectin from bovine plasma
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Affinity purified antibody lyophilized from phosphate buffered solution (non-sterile) . Contains antimycotic substance, but no BSA and sodium azide! Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C. For further dilution use appropriate antibody diluent.
Concentration
App. 1 mg/ml
Dilution
(purified, lyophilized) IFA 1:80, IHC (P) 1:500 (Envision), ELISA 1:200 (OD>500)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Bovine skin or liver. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC (P) 60 min at RT or 2-8°C over night
Tested Applications
ELISA, IF, IHC-P, RIA
Host or Source
Rabbit
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