Pan-Cytokeratin, 2 Clones (AE1/AE3) Antibody
Mouse monoclonal antibody to pan Cytokeratin
Product Specifications
Reactivity
Human
Immunogen
Human epithelial keratins
Clonality
Monoclonal
Clone
AE1/AE3
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 μl working solution / test) . Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Concentration
70 μg/ml
Dilution
(liquid conc.) 1:20 - 1:50
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Appendix. Pre-Treatment: Paraffin sections may be pre-treated with 1 % protease solution (Unmasking fluid P, Art.-No. DE110) for 15 min at RT, improvement of staining may be achieved with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000) . Incubation Time: 60 min at RT
Tested Applications
IHC-Fr, IHC-P
Host or Source
Mouse
Isotype
IgG1
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