ImmunoHistozyme

1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer. 2. Remove buffer, add 2-4 drops of ImmunoHistoZyme reagent to cover tissue section. 3. Incubate at room temp. for 5 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5 minutes are sufficient at room temp. In some cases it may require approximately10 minutes at room temp). 4. Discard ImmunoHistoZyme reagent, wash 3-5 times with buffer. 5. The tissue sections are ready for further IHC protocol.