MiRNA Agomir Negative Control

Synthetic miRNA Agomir negative control for experiments using targeted miRNA transfection

Product Specifications

Source

Synthetic

Form

Liquid

Storage Conditions

Shipped at 4 °C. Store at -20 °C for one year. Avoid freeze-thaw cycles after reconstitution.

Notes

For research use only.

Applications Notes

Components: This synthetic miRNA is based on the mature miRNA sequence. It does not contain the full precursor miRNA stem-loop. Directions for Use: Briefly centrifuge tubes containing miRNA agomir to ensure that the miRNA pellet is located at the bottom of the tube. Dissolve miRNA agomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water) . For example: dissolve 5 nmol miRNA agomir to 20 μM using 250 μl DEPC H2O (or RNase-free water) . Pipette the solution up and down 3-5 times (or vortex briefly) . Briefly centrifuge tubes containing miRNA agomir to ensure that the solution is collected at the bottom of the tube. Aliquot the miRNA agomir into small volumes and store at ≤ -20°C. miRNA agomir is stable (for 6 months under the specified storage condition) . For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times