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Mouse Human Bence Jones kappa (surface and hidden determinants) Antibody

Purified monoclonal mouse antibody to human Ig kappa chain, free and bound. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay- related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Product Specifications

Reactivity

Human

Clonality

Monoclonal

Clone

NI 285, NI 250

Conjugation

Unconjugated

Source

Highly purified Bence Jones kappa proteins isolated from pooled human urine.

Form

Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. No foreign proteins added.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Host or Source

Mouse

Isotype

IgG1

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