HCoV-229E Spike protein, His Tag

HCoV-229E Spike Protein, His Tag (SPN-H52H3) is expressed from human 293 cells (HEK293) . It contains AA Cys 16 - Pro 1115 (Accession # P15423-1 (TI 871-872 PP) ) .The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus. Proline substitutions TI 871-872 PP are introduced to stabilize the trimeric prefusion state of the spike protein.

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 125.7 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P15423-1

Expression Region

Cys 16 - Pro 1115

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

HCoV-229E

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.