HCoV-229E Spike protein, His Tag
HCoV-229E Spike Protein, His Tag (SPN-H52H3) is expressed from human 293 cells (HEK293) . It contains AA Cys 16 - Pro 1115 (Accession # P15423-1 (TI 871-872 PP) ) .The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus. Proline substitutions TI 871-872 PP are introduced to stabilize the trimeric prefusion state of the spike protein.
Product Specifications
Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 125.7 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P15423-1
Expression Region
Cys 16 - Pro 1115
Host
HEK293
Target
Spike protein
Conjugation
Unconjugated
Tag
C-10xHis
Source
HCoV-229E
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Format
Powder
Buffer
0.1 M Sodium citrate, pH5.5
Additives
Trehalose
Molecular Weight
125.7 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
500ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/SPN-H52H3.pdf
Species
HCoV-229E
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