Mca-SEVNLDAEFK (Dnp) -NH2

Mca-SEVNLDAEFK (Dnp) -NH2 contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence, such as can be used to measure the activity of BACE-1[1].

Product Specifications

CAS Number

[1802078-32-5]

UNSPSC

12352209

Target

Fluorescent Dye

Type

Peptides

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/mca-sevnldaefk-dnp-nh2.html

Purity

98.82

Solubility

DMSO : 1.25 mg/mL (ultrasonic)

Smiles

[O-][N+](C(C=C(C=C1)[N+]([O-])=O)=C1NCCCC[C@@H](C(N)=O)NC([C@H](CC2=CC=CC=C2)NC([C@H](CCC(O)=O)NC([C@H](C)NC([C@H](CC(O)=O)NC([C@H](CC(C)C)NC([C@H](CC(N)=O)NC([C@H](C(C)C)NC([C@H](CCC(O)=O)NC([C@H](CO)NC(CC(C3=CC=C(OC)C=C3O4)=CC4=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O

Molecular Formula

C68H89N15O26

Molecular Weight

1532.52

References & Citations

[1]Koike H, et.al. Thimet oligopeptidase cleaves the full-length Alzheimer amyloid precursor protein at a beta-secretase cleavage site in COS cells. J Biochem. 1999 Jul;126 (1) :235-42.