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Phi29 DNA Polymerase

Phi29 DNA polymerase is a recombinant protein purified from E.coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage Phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase. Phi29 DNA polymerase contains a 3'→5' exonuclease activity that enables proofreading capability.

Product Specifications

Product Name Alternative

Bacillus subtilis phage phi29, recombinant, E. coli

Field of Research

Molecular Biology

Concentration

10 units/μl

Form

Liquid

Storage Conditions

Store at -20 °C. avoid freeze/thaw cycles

Notes

For research use only.

Applications Notes

Rolling-circle amplification (RCA) ; Multiple displacement amplification (MDA) ; Whole genome amplification (WGA) ; Preparation of DNA template for sequencing. Inactivation: Heat the mixture to 65 °C for 15 min.

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