Phi29 DNA Polymerase
Phi29 DNA polymerase is a recombinant protein purified from E.coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage Phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase. Phi29 DNA polymerase contains a 3'→5' exonuclease activity that enables proofreading capability.
Product Specifications
Product Name Alternative
Bacillus subtilis phage phi29, recombinant, E. coli
Field of Research
Molecular Biology
Concentration
10 units/μl
Form
Liquid
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Rolling-circle amplification (RCA) ; Multiple displacement amplification (MDA) ; Whole genome amplification (WGA) ; Preparation of DNA template for sequencing. Inactivation: Heat the mixture to 65 °C for 15 min.
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