Phosph-Free Block ELISA Blocking Buffer
Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Phosph-Free Block is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.
Product Specifications
Concentration
1 X
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Add the antibody-AP or protein-AP conjugate directly into the Alkaline Phosphatase Conjugate Stabilizer to a working 1X dilution and mix for 5-15 minutes. 2. Pipette diluted AP conjugate onto the plate or store protected from light at 2-8°C for use at a later date. 1X working AP conjugate dilutions can be stored at 2-8°C and protected from light providing a useful shelf-life of 6 months.
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