Plasma Sample Diluent
Plasma Sample Diluent is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This novel mammalian-protein-buffered formulation provides an assay environment that minimizes non-specific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. Plasma Sample Diluent also inhibits thrombin activity (clotting) and complement activity during the plasma sample incubation period.
Product Specifications
Concentration
1 X
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
When diluting test samples, first note the dilution factor for future data analysis. For example, a human plasma sample was diluted 1:10 (50 μL plasma sample + 450 μL plasma sample diluent) and yielded a standard curve derived analyte concentration of 50 ng/mL from the ELISA assay. This concentration should then be multiplied by 10 (the dilution factor) to obtain the actual concentration value of 500 ng/mL in the undiluted plasma sample.
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