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Recombinant Human CD40

Product Specifications

Product Name Alternative

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

UniProt

P25942

Expression Region

21-193

Host

CHO cells

Tag

C-terminal His Tag

Applications

Western Blot, ELISA

Field of Research

Immunology, Signal Transduction

Endotoxin

< 1 EU/µg as determined by LAL test.

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA.Immobilized recombinant human CD40 at 1 μg/mL. (100 μl/well) can bind biotinylated human CD40L with a linear range of 25-600 ng/mL.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

32 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

Formulation

Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

Organism Species

Human

Available Sizes

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