What is the catalog number for Pronase Reagent (ready to use)?

The catalog number for Pronase Reagent (ready to use) is orb2652914.

What sizes are available for Pronase Reagent (ready to use)?

Pronase Reagent (ready to use) is available in the following size(s): 100 mL.

Is Pronase Reagent (ready to use) in stock?

Pronase Reagent (ready to use) is currently made to order / not held in immediate stock. Contact us for lead time.

Pronase Reagent (ready to use)

Pronase is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

Product Specifications

Storage Conditions

2-8 °C; DO NOT FREEZE

Notes

For research use only.

Applications Notes

1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer. 2. Remove buffer, add 2-4 drops of Pronase reagent to cover tissue section. 3. Incubate at room temp. for 5 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5 minutes are sufficient at room temp. In some cases it may require 15 minutes at room temp) . 4. Discard Pronase reagent, wash 3-5 times with buffer. 5. The tissue sections are ready for further IHC protocol.