Protein A Alkaline Phosphatase Conjugated
Protein A Alkaline Phosphatase Conjugated
Product Specifications
Product Name Alternative
ProA, Staphylococcus A protein, ALP, Protein A Alk Phos
Conjugation
AP
Applications
ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Purity
Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A. Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. Protein A Alkaline Phosphatase Conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Protein A Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
Tested Applications
DOT, ELISA, IHC, WB
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
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