Protein G Biotin Conjugated

Product Specifications

Product Name Alternative

ProG, Streptococcus G protein, Protein G Biotin Conjugated

Conjugation

Biotin

Applications

ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000

Concentration

Purity

Protein G Biotin Conjugated was prepared from chromatographically pure recombinant Protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein G. No reaction was observed against anti-Protein A.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Protein G Biotin Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype.

Tested Applications

ELISA, IHC, WB

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2