Protein Molecular Weight Marker

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Protein Molecular Weight Marker is supplied in 1X loading buffer and is ready-to-use. It contains: [62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue.] This product are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining) . The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference.

Protein Molecular Weight Marker has been tested and is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10μl is appropriate for most gel formats

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 2.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue