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PU.1 Antibody / SPI1

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity) . [UniProt]

Product Specifications

Reactivity

Human

Applications

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml

Field of Research

Stem Cell & Developmental Biology

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PU.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Tested Applications

IF, IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

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