RBM1-151

RBM1-151 is a 1-deoxy derivative and vinyl analog of RBM14-C12, serving as a fluorescent substrate for amidases (Ex/Em = 355/460 nm) . It is hydrolyzed by acidic ceramidase (AC) (appKm = 7.0 μM; appVmax = 99.3 nM/min), N-acylethanolamine hydrolyzing acid amidase (appKm = 0.73 μM; appVmax = 0.24 nM/min), and fatty acid amide hydrolase (FAAH) (appKm = 3.6 μM; appVmax = 7.6 nM/min), but is not hydrolyzed by other ceramidases. RBM1-151 is useful for studying the basic biology of lipid amidase functions and for potential diagnostic/prognostic assessments in disorders involving AC, NAAA, or FAAH dysregulation, such as Farber disease and cancer.