Anti-RFP | Red flourescent protein (mRFP, mCherry, tdTomato, mScarlet)
Selected Variant
AS24 5006
Size: 50 µg
Product Specifications
Background
Red fluorescent protein (RFP) once excited is fluorescing red-orange light. The mass of RFP is approximately 25.9 kDa and its excitation maximum is 558 nm and emission maximum is 583 nm. Improved variants of RFP include so called mFruits variants: mCherry, mOrange, mRaspberry.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
RFP, mCherry-YFP
Not reactive in
Sequence identity with GFP is very low and it is therefore expected that this antibody does not cross-reacti with GFP protein.
Immunogen
Recombinant protein produced in E.coli corresponding to full lenght mRFP. The sequence used for immunization is also found in other red flourescent proteins like: mCherry/tdTomato/mScarlet and others.
Clonality
Polyclonal
Applications
Immunohistochemistry (IHC), Western blot (WB)
Field of Research
BFP/FITC/GFP/RFP/TRITC/YFP
Dilution
1: 200 (IHC), 1: 2500 (WB)
Purity
Antigen affinity purified serum, in PBS pH 7.4
Format
Lyophilized
Reconstitution
For reconstitution, add 50 µl, of sterile or deionized water.
Molecular Weight
Depends upon fusion partner
References & Citations
To be added when available. Antibody released in February 2025.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Prediction Reactivity
MRFP, mCherry, tdTomato, mScarlet
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