Sepharose Protein A
Sepharose Protein A
Product Specifications
Product Name Alternative
ProA, Staphylococcus A protein, Sepharose Protein A
Conjugation
Sepharose
Applications
IP: User Optimized
Concentration
6.0 mg/cc
Purity
Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
Form
Suspension of agarose beads
Storage Conditions
Store SEPHAROSE PROTEIN A at 4° C prior to opening. DO NOT FREEZE.
Notes
For research use only.
Applications Notes
Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 μL per immunoprecipitation reaction.
Tested Applications
IP
Preservative
Preservative: None. Stabilizer: None; Buffer: 20% (v/v) Ethanol
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