Sepharose Protein A

Product Specifications

Product Name Alternative

ProA, Staphylococcus A protein, Sepharose Protein A

Conjugation

Sepharose

Applications

IP: User Optimized

Concentration

6.0 mg/cc

Purity

Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.

Form

Suspension of agarose beads

Storage Conditions

Store SEPHAROSE PROTEIN A at 4° C prior to opening. DO NOT FREEZE.

Notes

For research use only.

Applications Notes

Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 μL per immunoprecipitation reaction.