Streptavidin

Product Specifications

Product Name Alternative

SA, S avidin, streptococcus avidin

Conjugation

Unconjugated

Applications

ELISA: 1:20,000 - 1:200,000, WB: 1:2000-1:20,000

Field of Research

Non-Animal

Concentration

0.9 mg/mg

Purity

Streptavidin is chromatographically pure and shows predominantly a single 53,600 dalton band by SDS-PAGE.

Bioactivity

15.5 U/mg by biotin titration method

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Streptavidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized streptavidin.