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Streptavidin Alkaline Phosphatase Conjugated

Streptavidin Alkaline Phosphatase Conjugated

Product Specifications

Product Name Alternative

SA alkaline phosphatase conjugate, S avidin conjugated to alkaline phosphatase, alkaline phosphatase conjugated to streptococcus avidin, streptavidin Alk Phos, SA-ALP, ALP conjugated streptavidin

Conjugation

AP

Applications

ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:4,000

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Purity

Streptavidin-Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin. Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Streptavidin Alkaline Phosphatase conjugated has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10) .

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

CAA00084.1

Preservative

Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

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