Streptavidin Conjugated with Peroxidase
Streptavidin is a 52-66 kDa (average 60 KDa) (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC) .
Product Specifications
Applications
WB: 1:500-1:5,000. ICC: 1:200-1:1:1,000
Storage Conditions
2-8 °C
Notes
For research use only.
Applications Notes
This product can be diluted in our Immuno Automation buffer IBSC or PBS. BSA 0.5-1.0% should be added in these solutions. This product can also be diluted in our Universal antibody dilution buffer without adding any carrier protein.
Tested Applications
ELISA, ICC, IHC, WB
Preservative
The conjugate (one mg of streptavidin conjugated with peroxidase) is supplied in one ml of 10 mM PO4, 200 mM NaCl, pH 7.5, rAlbumin 10 mg/ml and 0.05% Proclin 300.
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