Streptavidin Peroxidase Conjugated
Streptavidin, which is obtained from the bacterium Streptomyces avidinii, exhibits an extraordinarily high binding affinity for biotin (B7) .
Product Specifications
Product Name Alternative
HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin
Conjugation
HRP
Applications
ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000
Field of Research
Non-Animal
Concentration
1.0 mg/mL
Purity
Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Western Blotting, and ELISA experiment formats in combination with the proper substrate.
Tested Applications
DOT, ELISA, IHC, WB
NCBI Accession Number
CAA00084.1
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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