Anti-ATM Antibody

Mouse Monoclonal Antibody specific to ATM

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.7.11.1, Ataxia telangiectasia mutated, A-T mutated

Gene Name

ATM

Gene ID

472

NCBI Gene ID

UniProt

Q13315

Accession Number

NP_000042

Cellular Locus

Nucleus, Cytoplasmic vesicle, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Host

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

GST fusion protein expressed in E. coli corresponding to aa 2577- 3056 of full-length ATM.

Target Antigen

Serine-protein kinase ATM

Target

ATM

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP, IHC

Field of Research

Cancer Research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. RPubMed:10550055, PubMed:10766245, PubMed:10802669, PubMed:10839545, PubMed:10910365, PubMed:10973490, PubMed:11375976, PubMed:12086603, PubMed:12556884, PubMed:14871926, PubMed:15448695, PubMed:15456891, PubMed:15916964, PubMed:16086026, PubMed:16858402, PubMed:17923702, PubMed:19431188, PubMed:19965871, PubMed:26774286, PubMed:29203878, PubMed:30612738, PubMed:30886146, PubMed:9733514, PubMed:9733515, PubMed:9843217}.

Additionnal Information

Immunoblotting: use at 1-10 ug/mL. <br><br>Immunoprecipitation: use at 1-10 ug/mL. <br><br>Positive controls: Raji or Akata cells.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length ATM, a 370 kD nuclear phosphoprotein, which is involved in the autooal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigations.