TMB ELISA Substrate
TMB ELISA Substrate detects horseradish peroxidase (HRP) activity.
Product Specifications
Field of Research
Cancer Biology
Form
Liquid
Storage Conditions
Store at 4 °C in dark for 1 year, do not freeze.
Notes
For research use only.
Applications Notes
(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20. (b) Add 100 μl of TMB solution/well. Incubate for a specific amount of time or until desired signal is achieved. (c) Add 100 μl of ELISA Stop Solution. (d) Read at Absorbance 450 nm.
Tested Applications
ELISA
Available Sizes
Frequently Asked Questions
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