Tris-HCL Buffer, 10X
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Product Specifications
Field of Research
Signal Transduction
Concentration
10 X
Storage Conditions
2-8 °C; DO NOT FREEZE
Notes
For research use only.
Applications Notes
1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or our Universal Immuno buffer. 2. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100oC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes. 3. The tissue sections are ready for further IHC protocol.
Tested Applications
IHC
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