Tris-HCL Buffer, 10X

1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or our Universal Immuno buffer. 2. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100oC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes. 3. The tissue sections are ready for further IHC protocol.