Triton X-100
Non ionic detergents like Triton X100 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background.
Product Specifications
Storage Conditions
Room Temperature
Notes
For research use only.
Applications Notes
For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml) . Washing buffer use 1:500 (1 ml into 499 ml distilled or deionized water) . The optimum dilution should be determined by the individual lab. (NOT RECOMMENDED FOR DILUTION OF S100 ANTIBODIES)
Tested Applications
ELISA, IHC
Preservative
This detergent is supplied as 50% in water with 0.15 % preservative.
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