Wash Buffer (20x), 500 ml (for 10 liter)

The solution should be stored at room temperature. It is stable up to the expiry date indicated on the label if undiluted. Do not use product after the expiry date. The diluted working strength solution is stable for about 1 week depending on the ambient temperature. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by this reagent, please contact our technical support.

The Wash Buffer is a 20fold concentrated phosphate buffer with additives of sodium chloride, detergent, and stabilising substances. For preparation of the working strength solution the buffer concentrate is diluted 1:20 with deionised or distilled water. The resulting solution has a pH of 7.2 (7.0 to 7.4) . The Wash Buffer • is wetting the tissue sections with detergent and thus reduces surface tension and improves spreading the reagents on the tissue section, • reduces unspecific binding of reagents on the tissue sample, • and because of the exact tuned salt concentration effects an excellent preservation of cell morphology.