Yeast Poly (A) Polymerase

Yeast Poly (A) Polymerase catalyzes the transfer of AMP to 3'-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+or Mn2+as cofactor. It works more efficiently than E.coli Poly (A) polymerase in some poly (A) tailing and RNA labeling reactions (e.g. shorter incubation time, broader acceptance of RNA template size) . Polyadenylation increases (m) RNA stability and therefore translation efficiency in transfection and microinjection experiments in eukary-otic cells. For information on (m) RNA polyadenylation using in vitro tran-scription reaction mixes as template, refer to the Poly (A) Tailing Enzyme Testkit (#orb611231) .