λDNA/ BstEII Digest
The BstEII digest of λ DNA yields the following discrete fragments (in base pairs) : 8453*, 7242, 6369, 5687*, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224, 117. The cohesive ends (12b cos site of bacteriophage) of fragments 8453 bp and 5687 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.
Product Specifications
Product Name Alternative
Ready-to-use
Concentration
200 ng/μl
Form
Liquid (supplied in 6x Green Gel Loading Buffer)
Storage Conditions
Store at -20 °C. Short term storage (up to 1 month) at 4 °C possible
Notes
For research use only.
Applications Notes
Preparation: Lambda DNA (48.502 bp) was completely digested by BstEII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Recommended Load: 5 μl per lane.
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