What is the catalog number for λDNA/ PstI Digest?

The catalog number for λDNA/ PstI Digest is orb1734264.

What sizes are available for λDNA/ PstI Digest?

λDNA/ PstI Digest is available in the following size(s): 1 mL.

Is λDNA/ PstI Digest in stock?

λDNA/ PstI Digest is currently made to order / not held in immediate stock. Contact us for lead time.

λDNA/ PstI Digest

The PstI digest of λ DNA yields the following discrete fragments (in base pairs) : 11501*, 5077, 4749, 4507, 2838, 2556*, 2459, 2443, 2140, 1986, 1700, 1159 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72, 15. The cohesive ends (12b cos site of bacteriophage) of fragments 11501 bp and 2556 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.

Product Specifications

Product Name Alternative

Ready-to-use

Concentration

100 ng/μl

Form

Liquid, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 8% (w/v) Glycerol, Tartrazine and Xylene Cyanol FF; Color: green

Storage Conditions

Store at -20 °C. Short term storage (up to 1 month) at 4 °C possible

Notes

For research use only.

Applications Notes

Preparation: Lambda DNA (48.502 bp) was completely digested by PstI, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Recommended Load: 5 μl per lane.