λDNA/ PstI Digest
The PstI digest of λ DNA yields the following discrete fragments (in base pairs) : 11501*, 5077, 4749, 4507, 2838, 2556*, 2459, 2443, 2140, 1986, 1700, 1159 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72, 15. The cohesive ends (12b cos site of bacteriophage) of fragments 11501 bp and 2556 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.
Product Specifications
Product Name Alternative
Ready-to-use
Concentration
100 ng/μl
Form
Liquid, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 8% (w/v) Glycerol, Tartrazine and Xylene Cyanol FF; Color: green
Storage Conditions
Store at -20 °C. Short term storage (up to 1 month) at 4 °C possible
Notes
For research use only.
Applications Notes
Preparation: Lambda DNA (48.502 bp) was completely digested by PstI, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Recommended Load: 5 μl per lane.
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