TMB Slow Kinetic 1-Component HRP Microwell Substrate (SUBK)
A ready-to-use solution containing TMB in a mildly acidic buffer. Ideal for ELISAs where the test samples contain high levels of the target molecule, for assays with long incubation periods, and for assays that do not need a high level of sensitivity.
Product Specifications
Field of Research
Cancer Biology
Concentration
1 X
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Allow product to equilibrate to room temperature (25°C) prior to use. 2. Add 100 μL to each well. When reacting with peroxidase, a soluble blue reaction product develops that can be read at 405-410 nm. 3. In endpoint assays, the reaction can be stopped by adding equal volumes of 1% sodium dodecyl sulfate (SDS) and reading at 405-410 nm.
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