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Goat Mouse IgG1 (subclass specific) Antibody

IgG fraction of polyclonal goat antiserum to Mouse IgG1, subclass specific. As unlabelled primary or secondary reagent for indirect detection of IgG1 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user's own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5.000.

Product Specifications

Reactivity

Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Source

Pools of purified homogenous IgG1 isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Cell Biology

Form

Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Host or Source

Goat

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