Sheep Mouse IgG2a (1b allotype specific) Antibody

Purified IgG fraction of polyclonal sheep antiserum to Mouse IgG2a (1b), allotype specific. As unlabelled primary or secondary reagent for indirect detection of IgG2a (1b) at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user's own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:4.000. These data should be interpreted as general recommendations only.