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Sheep Mouse Ig kappa light chains (free and bound) Antibody

IgG fraction of polyclonal sheep antiserum to free and bound mouse Ig kappa light chain. This IgG preparation is intended for use in precipitating (gel diffusion) and non- precipitating antibody-binding immunoassays (indirect immunofluorescence, enzyme immunoassays for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the kappa type as well as free light chains or Bence Jones proteins of the kappa type in serum or other body fluids.; to prepare an insoluble immunoaffinity adsorbent or a solid phase catching antibody reagent by coupling to an artificial carrier; for labeling with a marker of the user's own choice. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Working dilutions fro histochemical and cytochemical use are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Product Specifications

Reactivity

Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Source

A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Immunology & Inflammation

Form

Purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Host or Source

Sheep

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