Tyrosinase-IN-48

Tyrosinase-IN-48 (Compound 3) is a potent and competitive chalcone-type inhibitor of tyrosinase, with an IC50 value of 0.49 μM. It exhibits significant antioxidant potential, effectively scavenging DPPH and ABTS free radicals. Tyrosinase-IN-48 chelates the dinuclear copper ions at the active center of tyrosinase and reduces Cu2+ to Cu+, thereby diminishing the enzyme's catalytic activity. It demonstrates low cytotoxicity to HEK293 cells and zebrafish embryos. Tyrosinase-IN-48 also possesses anti-browning properties, which can improve food quality and is applicable in studies related to food preservation.