Anti-Estrogen Receptor beta Antibody

Mouse Monoclonal Antibody specific to Estrogen Receptor beta

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

ER-beta, Nuclear receptor subfamily 3 group A member 2

Gene Name

ESR2

Gene ID

2100

NCBI Gene ID

UniProt

Q92731

Accession Number

NP_001035365

Cellular Locus

Nucleus

Host

Mouse

Reactivity

Human

Immunogen

6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli.

Target Antigen

Estrogen receptor beta

Target

Estrogen Receptor beta

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, ICC/IF, ChIP, IHC

Field of Research

Cancer Research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). {PubMed:20074560, PubMed:29261182, PubMed:30113650, PubMed:9325313}.; [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. {PubMed:9671811}.

Additionnal Information

Immunoblotting: use at 1-5 ug/mL. <br><br>Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human ER-b, a novel receptor (approx. 52 kD) recently isolated from testis. The size and structure of ER-b are very similar to ER-alpha with the ligand and DNA binding domains being highly conserved. The N-terminus of each receptor, which serves as their transactivation domain, are significantly different. Like ER-alpha, ER-b binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements.