RNAP-σ interaction-IN-4

RNAP-σ interaction-IN-4 (Compound 3a) is an inhibitor of the interaction between RNA polymerase and the sigma factor (RNAP-σ) . It has a minimum inhibitory concentration (MIC) of 1 μg/mL against S. pneumoniae and 2 μg/mL against S. aureus. This compound exhibits potent bactericidal properties by disrupting bacterial transcription through interference with the β′CH−σ interaction. Additionally, RNAP-IN-2 has demonstrated significant efficacy in a sepsis model. RNAP-σ interaction-IN-4 is applicable for studying Gram-positive bacterial infections involving multidrug-resistant strains.