Tubulin polymerization-IN-85

Tubulin polymerization-IN-85 (Compound C21) is a microtubule polymerization inhibitor with an IC50 value of 1.59 μM, targeting the colchicine binding site. It exhibits antiproliferative activity against HeLa, HepG2, MCF-7, and A549 cells with IC50 values of 0.08, 0.21, 0.27, and 0.32 μM, respectively, while demonstrating low toxicity to normal renal epithelial 293T cells (IC50 value of 187 μM) . Tubulin polymerization-IN-85 induces G2/M phase arrest and triggers apoptosis in cancer cells. It downregulates the expression of Bcl-2, Bcl-xl, Mcl-1, Cyclin B1, cdc25, and cdc2 proteins, and upregulates P53, P21, Bad, and Bax levels. This compound is applicable in cancer research, such as cervical cancer studies.