WEE1-IN-14

WEE1-IN-14 (Compound 14) is a selective WEE1 inhibitor, with an IC50 of 0.5 nM determined by L-RB-FEP calculations and 1.0 nM in ADP-Glo​​ kinase assays. Inhibition of Wee1 in cancer cells disrupts the G2-M checkpoint, removing cell cycle regulation, leading to premature mitotic failure and subsequent tumor cell apoptosis. Thus, WEE1-IN-14 serves as a valuable tool for studying cancer biology.