Ferroptosis inducer-9

Ferroptosisinducer-9 is an agent that induces ferroptosis and acts as a microtubule polymerization inhibitor at the colchicine binding site. It inhibits MCF-7 cell growth with an IC50 of 14 nM and reduces [3H]colchicine binding. The compound decreases GPX4 and FTH expression while increasing COX2 and ACSL4 levels; it also lowers GSH, NADP+, and NADPH, and raises LPO, MDA, and Fe (II) concentrations, alongside reducing SOD levels. Ferroptosisinducer-9 demonstrates significant antitumor efficacy in HCT116 CRC xenograft models and is applicable in the study of triple-negative breast cancer (TNBC) and colorectal cancer (CRC) .