DNA/TOP2A-IN-1

DNA/TOP2A-IN-1 functions as an inhibitor of DNA and TOP2A. It binds selectively to TOP2A instead of TOP2B and interacts with DNA to form a stable DM1-TOP2A-DNA ternary complex. This compound induces DNA damage, elevates reactive oxygen species (ROS), and triggers apoptosis in triple-negative breast cancer (TNBC) cells. Additionally, DNA/TOP2A-IN-1 disrupts microtubule distribution and induces cell cycle arrest while exhibiting potent antiproliferative activity and inhibiting cell migration. It effectively suppresses tumor growth and is a valuable agent for TNBC research.