D574-0246

D574-0246 is a dual-active inhibitor of OXCT1, targeting the enzyme's keto-dehydrogenase and succinyl-transferase activities. It reduces substrate-specific (LACTB K284) and global protein succinylation in HepG2 cells and lowers the ketolytic activity of OXCT1. The compound decreases the viability of HCC cells with an IC50 of 16.49 μM in PLC cells and 6.656 μM in HepG2 cells. In nude mice with HepG2 xenograft tumors overexpressing OXCT1, D574-0246 demonstrates anti-tumor effects. It is applicable for studies on hepatocellular carcinoma (HCC) .