Carbonic anhydrase-IN-35

Carbonic anhydrase-IN-35 is a selective inhibitor of carbonic anhydrase (CA), demonstrating potent inhibition of tumor-associated hCA IX (Ki= 0.6 nM) and hCA XII (Ki= 2.2 nM) . It induces autophagic apoptosis in MCF-7 cells by increasing Bax, decreasing Bcl-2, and downregulating CDK4/6. Carbonic anhydrase-IN-35 exhibits significant cytotoxicity against MCF-7 (IC50= 0.3975 μM normoxia / 0.6575 μM hypoxia), MCF-7-ADR (IC50= 0.3975 μM normoxia / 4.488 μM hypoxia), MDA-MB-231, and 4T1 breast cancer cells. This compound is applicable in breast cancer research.