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Recombinant Human AMY1A Protein (His Tag)

Product Specifications

Background

Calcium-binding enzyme that initiates starch digestion in the oral cavity. Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins.

Abbreviation

AMY1A

Synonyms

Amy;4-alpha-D-glucan glucanohydrolase; AMY1A; AMY1;1; Alpha-amylase 1A;4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; EC:3.2.1.1

UniProt

P0DUB6

Accession Number

P0DUB6

Expression System

Mammalian

Tag

C-His

Sequence

Met1-Leu511

Field of Research

Metabolism; Neuroscience

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

56.1 kDa

Observed Molecular Weight

58 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human

Available Sizes

Curated Selection

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