Recombinant Human AMY1A Protein (His Tag)
Product Specifications
Background
Calcium-binding enzyme that initiates starch digestion in the oral cavity. Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins.
Abbreviation
AMY1A
Synonyms
Amy;4-alpha-D-glucan glucanohydrolase; AMY1A; AMY1;1; Alpha-amylase 1A;4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; EC:3.2.1.1
UniProt
P0DUB6
Accession Number
P0DUB6
Expression System
Mammalian
Tag
C-His
Sequence
Met1-Leu511
Field of Research
Metabolism; Neuroscience
Endotoxin
< 1.0 EU/mg of the protein as determined by the LAL method
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
56.1 kDa
Observed Molecular Weight
58 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
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